| 451 | North Butler Jr/Sr High School | Greene | North Butler Comm School District | 263 | 254 | -3.4% |
| 452 | Orange Elementary School | Waterloo | Waterloo Comm School District | 536 | 518 | -3.4% |
| 453 | Treynor Elementary School | Treynor | Treynor Comm School District | 379 | 366 | -3.4% |
| 454 | Alexander Elementary | Iowa City | Iowa City Comm School District | 452 | 436 | -3.5% |
| 455 | Woodland Hills Elementary School | West Des Moines | Waukee Comm School District | 599 | 578 | -3.5% |
| 456 | Audubon Elementary School | Dubuque | Dubuque Comm School District | 256 | 247 | -3.5% |
| 457 | Denison Elementary School | Denison | Denison Comm School District | 716 | 691 | -3.5% |
| 458 | Howe Elementary | Des Moines | Des Moines Independent Comm School District | 308 | 297 | -3.6% |
| 459 | Mediapolis Elementary School | Mediapolis | Mediapolis Comm School District | 448 | 432 | -3.6% |
| 460 | Runnells Elementary | Runnells | Southeast Polk Comm School District | 270 | 260 | -3.7% |
| 461 | Fillmore Elementary School | Davenport | Davenport Comm School District | 403 | 388 | -3.7% |
| 462 | Franklin Elementary School | Le Mars | Le Mars Comm School District | 271 | 261 | -3.7% |
| 463 | Thomas Jefferson Elementary School | Newton | Newton Comm School District | 395 | 380 | -3.8% |
| 464 | Prairie Crest Elementary | Cedar Rapids | College Comm School District | 451 | 434 | -3.8% |
| 465 | East Elementary School | Ankeny | Ankeny Comm School District | 425 | 409 | -3.8% |
| 466 | Prairie Ridge Elementary | Cedar Rapids | College Comm School District | 456 | 438 | -3.9% |
| 467 | Echo Hill Elementary | Marion | Linn-Mar Comm School District | 558 | 536 | -3.9% |
| 468 | Hillside Elementary School | West Des Moines | West Des Moines Comm School District | 571 | 548 | -4.0% |
| 469 | Moore Elementary School | Des Moines | Des Moines Independent Comm School District | 297 | 285 | -4.0% |
| 470 | Cherokee Elementary School | Cherokee | Cherokee Comm School District | 488 | 468 | -4.1% |
| 471 | Pineview Elementary | Iowa Falls | Iowa Falls Comm School District | 293 | 281 | -4.1% |
| 472 | West Elementary School | Emmetsburg | Emmetsburg Comm School District | 306 | 293 | -4.2% |
| 473 | Fairmeadows Elementary School | West Des Moines | West Des Moines Comm School District | 566 | 542 | -4.2% |
| 474 | Shenandoah High School | Shenandoah | Shenandoah Comm School District | 313 | 300 | -4.2% |
| 475 | North Tama High School | Traer | North Tama County Comm School District | 212 | 203 | -4.2% |
| 476 | Mid-Prairie Middle School | Kalona | Mid-Prairie Comm School District | 433 | 415 | -4.2% |
| 477 | King Elementary School | Des Moines | Des Moines Independent Comm School District | 329 | 315 | -4.3% |
| 478 | Buffalo Elementary School | Buffalo | Davenport Comm School District | 253 | 242 | -4.3% |
| 479 | Danville Junior-Senior High School | Danville | Danville Comm School District | 302 | 289 | -4.3% |
| 480 | Eddyville-Blakesburg-Fremont Jr/Sr High | Eddyville | Eddyville-Blakesburg- Fremont CSD | 451 | 431 | -4.4% |
| 481 | Sergeant Bluff-Luton Primary School | Sergeant Bluff | Sergeant Bluff-Luton Comm School District | 365 | 349 | -4.4% |
| 482 | Washington Middle School | Washington | Washington Comm School District | 363 | 347 | -4.4% |
| 483 | Lou Henry Elementary School | Waterloo | Waterloo Comm School District | 481 | 460 | -4.4% |
| 484 | Treynor Middle School | Treynor | Treynor Comm School District | 206 | 197 | -4.4% |
| 485 | Pleasant View Elementary School | Webster City | Webster City Comm School District | 220 | 210 | -4.5% |
| 486 | Lincoln Elementary School | Osage | Osage Comm School District | 424 | 405 | -4.5% |
| 487 | Pleasant View Elementary School | Bettendorf | Pleasant Valley Comm School District | 651 | 621 | -4.6% |
| 488 | Fairview Park Elementary | Spencer | Spencer Comm School District | 261 | 249 | -4.6% |
| 489 | Grant Wood Elementary School | Cedar Rapids | Cedar Rapids Comm School District | 373 | 356 | -4.6% |
| 490 | West Senior High School | Iowa City | Iowa City Comm School District | 1,587 | 1,514 | -4.6% |
| 491 | Bettendorf High School | Bettendorf | Bettendorf Comm School District | 1,503 | 1,433 | -4.7% |
| 492 | Thomas Jefferson High School | Cedar Rapids | Cedar Rapids Comm School District | 1,575 | 1,501 | -4.7% |
| 493 | Greene County High School | Jefferson | Greene County Comm School District | 394 | 375 | -4.8% |
| 494 | MOC-Floyd Valley Middle School | Alton | MOC-Floyd Valley Comm School District | 354 | 337 | -4.8% |
| 495 | Greene County Elementary | Jefferson | Greene County Comm School District | 544 | 518 | -4.8% |
| 496 | Algona Middle School | Algona | Algona Comm School District | 440 | 419 | -4.8% |
| 497 | Washington Elementary School | Mount Vernon | Mount Vernon Comm School District | 571 | 543 | -4.9% |
| 498 | Altoona Elementary | Altoona | Southeast Polk Comm School District | 451 | 429 | -4.9% |
| 499 | Urbandale Middle School | Urbandale | Urbandale Comm School District | 938 | 892 | -4.9% |
| 500 | Keokuk Middle School | Keokuk | Keokuk Comm School District | 401 | 381 | -5.0% |