| 401 | Riverdale Heights Elem School | Bettendorf | Pleasant Valley Comm School District | 636 | 628 | -1.3% |
| 402 | Glenwood Middle School | Glenwood | Glenwood Comm School District | 459 | 453 | -1.3% |
| 403 | Eleanor Roosevelt Middle School | Dubuque | Dubuque Comm School District | 1,095 | 1,081 | -1.3% |
| 404 | Cowles Elementary School | Des Moines | Des Moines Independent Comm School District | 397 | 392 | -1.3% |
| 405 | New London Jr-Sr High School | New London | New London Comm School District | 288 | 284 | -1.4% |
| 406 | Sunnyside Elementary School | Sioux City | Sioux City Comm School District | 248 | 244 | -1.6% |
| 407 | Dike Elementary School | Dike | Dike-New Hartford Comm School District | 311 | 306 | -1.6% |
| 408 | Greene County Middle School | Jefferson | Greene County Comm School District | 353 | 347 | -1.7% |
| 409 | West High School | Waterloo | Waterloo Comm School District | 1,725 | 1,696 | -1.7% |
| 410 | Wapsie Valley High School | Fairbank | Wapsie Valley Comm School District | 278 | 273 | -1.8% |
| 411 | Cascade Elementary School | Cascade | Western Dubuque Comm School District | 311 | 305 | -1.9% |
| 412 | Jefferson Elementary School | Muscatine | Muscatine Comm School District | 450 | 441 | -2.0% |
| 413 | Northeast Middle-High School | Goose Lake | Northeast Comm School District | 461 | 452 | -2.0% |
| 414 | Central Decatur MS/HS High School | Leon | Central Decatur Comm School District | 297 | 291 | -2.0% |
| 415 | Roland-Story Elementary School | Story City | Roland-Story Comm School District | 455 | 446 | -2.0% |
| 416 | Nevada Middle School | Nevada | Nevada Comm School District | 451 | 442 | -2.0% |
| 417 | Viola Gibson Elementary School | Cedar Rapids | Cedar Rapids Comm School District | 578 | 565 | -2.2% |
| 418 | Hoover Elementary School | West Branch | West Branch Comm School District | 361 | 353 | -2.2% |
| 419 | Eagle Heights Elementary School | Clinton | Clinton Comm School District | 453 | 443 | -2.2% |
| 420 | Decorah High School | Decorah | Decorah Community School District | 568 | 555 | -2.3% |
| 421 | Waverly-Shell Rock Middle School | Waverly | Waverly-Shell Rock Comm School District | 697 | 681 | -2.3% |
| 422 | Centennial Elementary | Altoona | Southeast Polk Comm School District | 433 | 423 | -2.3% |
| 423 | Fairview Elementary School | Grinnell | Grinnell-Newburg Comm School District | 248 | 242 | -2.4% |
| 424 | Pleasantville High School | Pleasantville | Pleasantville Comm School District | 247 | 241 | -2.4% |
| 425 | Moville Elementary School | Moville | Woodbury Central Comm School District | 290 | 283 | -2.4% |
| 426 | Pocahontas Area Middle/ High School | Pocahontas | Pocahontas Area Comm School District | 376 | 367 | -2.4% |
| 427 | North Fayette Valley High School | West Union | North Fayette Valley Comm School District | 331 | 323 | -2.4% |
| 428 | Central DeWitt Intermediate School | DeWitt | Central DeWitt School District | 319 | 311 | -2.5% |
| 429 | Spirit Lake High School | Spirit Lake | Spirit Lake Comm School District | 403 | 393 | -2.5% |
| 430 | Humboldt Middle School | Humboldt | Humboldt Comm School District | 466 | 454 | -2.6% |
| 431 | Feelhaver Elementary School | Fort Dodge | Fort Dodge Comm School District | 233 | 227 | -2.6% |
| 432 | Mount Ayr High School | Mount Ayr | Mount Ayr Comm School District | 312 | 304 | -2.6% |
| 433 | Mary Welsh Elementary | Williamsburg | Williamsburg Comm School District | 621 | 604 | -2.7% |
| 434 | North Middle School | Sioux City | Sioux City Comm School District | 1,168 | 1,136 | -2.7% |
| 435 | Central Lee Elementary School | Donnellson | Central Lee Comm School District | 580 | 564 | -2.8% |
| 436 | Prairie Creek | Cedar Rapids | College Comm School District | 870 | 846 | -2.8% |
| 437 | Webster Elementary School | Urbandale | Urbandale Comm School District | 534 | 519 | -2.8% |
| 438 | McKinley Elementary School | Des Moines | Des Moines Independent Comm School District | 283 | 275 | -2.8% |
| 439 | Van Buren Elementary School | Cedar Rapids | Cedar Rapids Comm School District | 442 | 429 | -2.9% |
| 440 | Perkins Elementary School | Des Moines | Des Moines Independent Comm School District | 451 | 438 | -2.9% |
| 441 | B R Miller Middle School | Marshalltown | Marshalltown Comm School District | 758 | 736 | -2.9% |
| 442 | Longfellow Elementary School | Iowa City | Iowa City Comm School District | 335 | 325 | -3.0% |
| 443 | Logan-Magnolia Jr-Sr High School | Logan | Logan-Magnolia Comm School District | 303 | 294 | -3.0% |
| 444 | Storm Lake Middle School | Storm Lake | Storm Lake Comm School District | 672 | 651 | -3.1% |
| 445 | Webster City High School | Webster City | Webster City Comm School District | 521 | 505 | -3.1% |
| 446 | Parkview Middle School | Ankeny | Ankeny Comm School District | 894 | 865 | -3.2% |
| 447 | West Marshall High School | State Center | West Marshall Comm School District | 302 | 292 | -3.3% |
| 448 | Crossroads Park Elementary School | West Des Moines | West Des Moines Comm School District | 548 | 530 | -3.3% |
| 449 | Boyer Valley Elementary School | Dow City | Boyer Valley Comm School District | 215 | 208 | -3.3% |
| 450 | MFL MarMac Elementary School | Monona | MFL MarMac Comm School District | 329 | 318 | -3.3% |