| 501 | Aplington-Parkersburg High School | Parkersburg | Aplington-Parkersburg Comm School District | 260 | 247 | -5.0% |
| 502 | Stewart Elementary School | Washington | Washington Comm School District | 481 | 457 | -5.0% |
| 503 | Maquoketa Community High School | Maquoketa | Maquoketa Comm School District | 502 | 477 | -5.0% |
| 504 | Jackson Elementary School | Davenport | Davenport Comm School District | 339 | 322 | -5.0% |
| 505 | Iowa Valley Jr-Sr High School | Marengo | Iowa Valley Comm School District | 241 | 229 | -5.0% |
| 506 | Camanche Elementary | Camanche | Camanche Comm School District | 377 | 358 | -5.0% |
| 507 | Harlan Community Middle School | Harlan | Harlan Comm School District | 353 | 335 | -5.1% |
| 508 | Newton Senior High School | Newton | Newton Comm School District | 850 | 806 | -5.2% |
| 509 | Harlan Intermediate School | Harlan | Harlan Comm School District | 326 | 309 | -5.2% |
| 510 | Lincoln Elementary School | Iowa City | Iowa City Comm School District | 229 | 217 | -5.2% |
| 511 | Hoover Elementary School | Cedar Rapids | Cedar Rapids Comm School District | 443 | 420 | -5.2% |
| 512 | Franklin Elementary School | Marshalltown | Marshalltown Comm School District | 378 | 358 | -5.3% |
| 513 | Wright Elementary School | Des Moines | Des Moines Independent Comm School District | 280 | 265 | -5.4% |
| 514 | Willowbrook Elementary | Altoona | Southeast Polk Comm School District | 553 | 523 | -5.4% |
| 515 | Northeast Elementary School | Glenwood | Glenwood Comm School District | 569 | 538 | -5.4% |
| 516 | Harlan High School | Harlan | Harlan Comm School District | 514 | 486 | -5.4% |
| 517 | East Elementary School | Independence | Independence Comm School District | 280 | 265 | -5.4% |
| 518 | Iowa Falls - Alden High School | Iowa Falls | Iowa Falls Comm School District | 421 | 398 | -5.5% |
| 519 | Findley Elementary School | Des Moines | Des Moines Independent Comm School District | 343 | 324 | -5.5% |
| 520 | Northwest Elementary School | Ankeny | Ankeny Comm School District | 366 | 346 | -5.5% |
| 521 | Clark Elementary School | Le Mars | Le Mars Comm School District | 319 | 301 | -5.6% |
| 522 | East Buchanan Elementary School | Winthrop | East Buchanan Comm School District | 321 | 303 | -5.6% |
| 523 | Wayne Community Jr-Sr High School | Corydon | Wayne Comm School District | 270 | 255 | -5.6% |
| 524 | Belmond-Klemme Community Jr-Sr High School | Belmond | Belmond-Klemme Comm School District | 315 | 297 | -5.7% |
| 525 | Roland-Story High School | Story City | Roland-Story Comm School District | 336 | 317 | -5.7% |
| 526 | Northeast Elementary | Ankeny | Ankeny Comm School District | 631 | 595 | -5.7% |
| 527 | East Union Middle-High School | Afton | East Union Comm School District | 257 | 242 | -5.8% |
| 528 | Van Buren County Community Middle & High School | Keosauqua | Van Buren County Comm School District | 376 | 354 | -5.9% |
| 529 | Earlham Senior High School | Earlham | Earlham Comm School District | 201 | 189 | -6.0% |
| 530 | Dubuque Senior High School | Dubuque | Dubuque Comm School District | 1,585 | 1,490 | -6.0% |
| 531 | Prairie View Elementary School | Cedar Rapids | College Comm School District | 458 | 430 | -6.1% |
| 532 | Broadway Elementary School | Denison | Denison Comm School District | 307 | 288 | -6.2% |
| 533 | Rock Creek Elementary | Ankeny | Ankeny Comm School District | 681 | 639 | -6.2% |
| 534 | Lake Mills Elementary School | Lake Mills | Lake Mills Comm School District | 323 | 303 | -6.2% |
| 535 | Waukee Elementary School | Waukee | Waukee Comm School District | 753 | 706 | -6.2% |
| 536 | Prairie City Elementary School | Prairie City | PCM Comm School District | 256 | 240 | -6.3% |
| 537 | Grant Ragan Elementary School | Waukee | Waukee Comm School District | 712 | 666 | -6.5% |
| 538 | Central Elementary School | Nevada | Nevada Comm School District | 676 | 632 | -6.5% |
| 539 | North Mahaska Elementary School | New Sharon | North Mahaska Comm School District | 322 | 301 | -6.5% |
| 540 | Valley Southwoods | West Des Moines | West Des Moines Comm School District | 725 | 678 | -6.5% |
| 541 | Prairie Hill Elementary School | Cedar Rapids | College Comm School District | 499 | 466 | -6.6% |
| 542 | Roosevelt Elementary School | Council Bluffs | Council Bluffs Comm School District | 487 | 455 | -6.6% |
| 543 | Charles City High School | Charles City | Charles City Comm School District | 506 | 472 | -6.7% |
| 544 | Baxter Elementary School | Baxter | Baxter Comm School District | 224 | 209 | -6.7% |
| 545 | George Washington Carver Academy | Waterloo | Waterloo Comm School District | 488 | 455 | -6.8% |
| 546 | Southeast Valley Middle School | Burnside | Southeast Valley Community School District | 337 | 314 | -6.8% |
| 547 | Boyer Valley Middle/High School | Dunlap | Boyer Valley Comm School District | 235 | 219 | -6.8% |
| 548 | Gladbrook-Reinbeck Jr. High & High School | Reinbeck | Gladbrook-Reinbeck Comm School District | 222 | 207 | -6.8% |
| 549 | Perry High School | Perry | Perry Comm School District | 589 | 549 | -6.8% |
| 550 | East Marshall Senior High School | Le Grand | East Marshall Comm School District | 233 | 217 | -6.9% |