| 551 | Lincoln Elementary School | Dubuque | Dubuque Comm School District | 276 | 257 | -6.9% |
| 552 | McKinley Elementary School | Muscatine | Muscatine Comm School District | 423 | 394 | -6.9% |
| 553 | Garner-Hayfield-Ventura High School | Garner | Garner-Hayfield-Ventura Comm School District | 317 | 295 | -6.9% |
| 554 | Blue Grass Elementary School | Blue Grass | Davenport Comm School District | 298 | 277 | -7.0% |
| 555 | Bloomer Elementary School | Council Bluffs | Council Bluffs Comm School District | 426 | 396 | -7.0% |
| 556 | Oskaloosa Elementary School | Oskaloosa | Oskaloosa Comm School District | 972 | 903 | -7.1% |
| 557 | McKinley STEAM Academy | Cedar Rapids | Cedar Rapids Comm School District | 459 | 426 | -7.2% |
| 558 | Westwood Elementary School | Sloan | Westwood Comm School District | 347 | 322 | -7.2% |
| 559 | Lake Mills Senior High School | Lake Mills | Lake Mills Comm School District | 208 | 193 | -7.2% |
| 560 | Neil Armstrong Elementary School | Bettendorf | Bettendorf Comm School District | 289 | 268 | -7.3% |
| 561 | Center Point-Urbana High School | Center Point | Center Point-Urbana Comm School District | 475 | 440 | -7.4% |
| 562 | Thomas Jefferson High School | Council Bluffs | Council Bluffs Comm School District | 1,237 | 1,146 | -7.4% |
| 563 | Hoover Middle School | Waterloo | Waterloo Comm School District | 916 | 848 | -7.4% |
| 564 | Edgewood-Colesburg Elementary School | Colesburg | Edgewood-Colesburg Comm School District | 307 | 284 | -7.5% |
| 565 | Vinton-Shellsburg Middle School | Vinton | Vinton-Shellsburg Comm School District | 358 | 331 | -7.5% |
| 566 | Vinton-Shellsburg High School | Vinton | Vinton-Shellsburg Comm School District | 448 | 414 | -7.6% |
| 567 | Monroe Elementary School | Monroe | PCM Comm School District | 278 | 257 | -7.6% |
| 568 | Moulton Elementary School | Des Moines | Des Moines Independent Comm School District | 504 | 465 | -7.7% |
| 569 | Clarion-Goldfield-Dows Elementary School | Clarion | Clarion-Goldfield-Dows Comm School District | 508 | 469 | -7.7% |
| 570 | Oelwein High School | Oelwein | Oelwein Comm School District | 362 | 334 | -7.7% |
| 571 | Sibley Ocheyedan Elementary School | Sibley | Sibley-Ocheyedan Comm School District | 334 | 308 | -7.8% |
| 572 | Sioux Center Middle School | Sioux Center | Sioux Center Comm School District | 398 | 367 | -7.8% |
| 573 | Bondurant - Farrar Intermediate School | Bondurant | Bondurant-Farrar Comm School District | 483 | 445 | -7.9% |
| 574 | Muscatine High School | Muscatine | Muscatine Comm School District | 1,502 | 1,380 | -8.1% |
| 575 | Carrie Lee Elementary | Decorah | Decorah Community School District | 234 | 215 | -8.1% |
| 576 | Weber Elementary | Iowa City | Iowa City Comm School District | 496 | 456 | -8.1% |
| 577 | Helen A Hansen Elementary School | Cedar Falls | Cedar Falls Comm School District | 619 | 569 | -8.1% |
| 578 | John F Kennedy High School | Cedar Rapids | Cedar Rapids Comm School District | 1,795 | 1,648 | -8.2% |
| 579 | Callanan Middle School | Des Moines | Des Moines Independent Comm School District | 603 | 553 | -8.3% |
| 580 | Kluckhohn Elementary School | Le Mars | Le Mars Comm School District | 444 | 407 | -8.3% |
| 581 | Storm Lake Elementary | Storm Lake | Storm Lake Comm School District | 896 | 822 | -8.3% |
| 582 | Manson Northwest Webster Elementary | Barnum | Manson Northwest Webster Comm School District | 479 | 439 | -8.4% |
| 583 | J C Hoglan Elementary School | Marshalltown | Marshalltown Comm School District | 368 | 337 | -8.4% |
| 584 | Burlington Community High School | Burlington | Burlington Comm School District | 1,109 | 1,016 | -8.4% |
| 585 | Stilwell Junior High School | West Des Moines | West Des Moines Comm School District | 705 | 645 | -8.5% |
| 586 | Iowa Valley Elementary School | Marengo | Iowa Valley Comm School District | 343 | 314 | -8.5% |
| 587 | McCombs Middle School | Des Moines | Des Moines Independent Comm School District | 733 | 670 | -8.6% |
| 588 | Pence Elementary | Fairfield | Fairfield Comm School District | 347 | 317 | -8.6% |
| 589 | Mount Pleasant High School | Mount Pleasant | Mount Pleasant Comm School District | 597 | 545 | -8.7% |
| 590 | Sidney Elementary School | Sidney | Sidney Comm School District | 264 | 241 | -8.7% |
| 591 | Wilton Elementary School | Wilton | Wilton Comm School District | 558 | 509 | -8.8% |
| 592 | Bellevue High School | Bellevue | Bellevue Comm School District | 388 | 354 | -8.8% |
| 593 | Eisenhower Elementary School | Davenport | Davenport Comm School District | 452 | 412 | -8.8% |
| 594 | Central High School | Davenport | Davenport Comm School District | 1,414 | 1,288 | -8.9% |
| 595 | Underwood Middle School | Underwood | Underwood Comm School District | 202 | 184 | -8.9% |
| 596 | Longfellow Elementary School | Belle Plaine | Belle Plaine Comm School District | 293 | 267 | -8.9% |
| 597 | Hanawalt Elementary | Des Moines | Des Moines Independent Comm School District | 343 | 312 | -9.0% |
| 598 | Gerald W Kirn Middle School | Council Bluffs | Council Bluffs Comm School District | 1,004 | 913 | -9.1% |
| 599 | Anamosa High School | Anamosa | Anamosa Comm School District | 419 | 381 | -9.1% |
| 600 | Paul Norton Elementary School | Bettendorf | Bettendorf Comm School District | 444 | 403 | -9.2% |